pegfp-n2 expression vector (TransGen biotech co)
90
Structured Review
TransGen biotech co
pegfp-n2 expression vector
Pegfp N2 Expression Vector, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pegfp-n2+expression+vector/pegfp+n2+expression+vector/pmc08018529-48-10-13
Average 90 stars, based on 1 article reviews
Pegfp N2 Expression Vector, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pegfp-n2+expression+vector/pegfp+n2+expression+vector/pmc08018529-48-10-13
Average 90 stars, based on 1 article reviews
pegfp-n2 expression vector - by Bioz Stars,
2026-10
90/100 stars
Images
1) Product Images from "Novel Compound Heterozygous Pathogenic Mutations of SLC5A5 in a Chinese Patient With Congenital Hypothyroidism"
Article Title: Novel Compound Heterozygous Pathogenic Mutations of SLC5A5 in a Chinese Patient With Congenital Hypothyroidism
Journal: Frontiers in Endocrinology
doi: 10.3389/fendo.2021.620117
Figure Legend Snippet: Characterization of cellular localization of G421R and G51fs NIS mutants in 293T cells. pEGFP-N2 empty vector was employed as a negative control (Mock). Human WT NIS, D331N, G421R, and G51fs mutants were cloned into pEGFP-N2 plasmids (green). The cell membrane was labeled with a fluorescent probe Dil (red). Mock, WT NIS, D331N, G421R, and G51fs plasmids were transfected into 293T cells 24 h after seeding. Mutant D331N NIS showed slightly decreased membrane fluorescence, compared with the WT. However, G51fs and G421R mutants displayed severely reduced cell membrane localization in 293T cells. Nuclei were stained with DAPI (blue). Scale: 10 μm.
Techniques Used: Plasmid Preparation, Negative Control, Clone Assay, Labeling, Transfection, Mutagenesis, Fluorescence, Staining
Related Articles
Expressing:Article Title: Caveolin-1 promotes the transformation and anti-apoptotic ability of mouse hepatoma cells. Article Snippet: Caveolin-1 is a major structural protein of caveolae and plays important roles in signal transduction, cellular transformation and tumor metastasis.. Our previous study demonstrated that caveolin-1 expression level was positively correlated with the invasive ability of mouse hepatoma Hepa1-6 and Hca-F cells.. However, the role of caveolin-1 in cellular transformation and apoptosis remains undetermined. Article Title: Functional Analyses of Glycyl-tRNA Synthetase Mutations Suggest a Key Role for tRNA-Charging Enzymes in Peripheral Axons Article Snippet: Alternatively, the PCR primers contained flanking Pst I (forward) and Sma I (reverse) restriction sites to allow cloning into the pEGFP-N2 expression vector (BD Biosciences, Palo Alto, CA) in frame with a C-terminal enhanced green fluorescent protein (EGFP) tag. Article Title: Novel Compound Heterozygous Pathogenic Mutations of SLC5A5 in a Chinese Patient With Congenital Hypothyroidism Article Snippet: Similarly, a WT NIS-EGFP fusion protein was expressed in a pEGFP-N2 expression vector (TransGen Biotech) using EcoRI and BamHI restriction sites, and the GFP was tagged downstream to the NIS coding region. Plasmid Preparation:Article Title: Caveolin-1 promotes the transformation and anti-apoptotic ability of mouse hepatoma cells. Article Snippet: Caveolin-1 is a major structural protein of caveolae and plays important roles in signal transduction, cellular transformation and tumor metastasis.. Our previous study demonstrated that caveolin-1 expression level was positively correlated with the invasive ability of mouse hepatoma Hepa1-6 and Hca-F cells.. However, the role of caveolin-1 in cellular transformation and apoptosis remains undetermined. Article Title: Functional Analyses of Glycyl-tRNA Synthetase Mutations Suggest a Key Role for tRNA-Charging Enzymes in Peripheral Axons Article Snippet: Alternatively, the PCR primers contained flanking Pst I (forward) and Sma I (reverse) restriction sites to allow cloning into the pEGFP-N2 expression vector (BD Biosciences, Palo Alto, CA) in frame with a C-terminal enhanced green fluorescent protein (EGFP) tag. Article Title: Novel Compound Heterozygous Pathogenic Mutations of SLC5A5 in a Chinese Patient With Congenital Hypothyroidism Article Snippet: Similarly, a WT NIS-EGFP fusion protein was expressed in a pEGFP-N2 expression vector (TransGen Biotech) using EcoRI and BamHI restriction sites, and the GFP was tagged downstream to the NIS coding region. Negative Control:Article Title: Caveolin-1 promotes the transformation and anti-apoptotic ability of mouse hepatoma cells. Article Snippet: Caveolin-1 is a major structural protein of caveolae and plays important roles in signal transduction, cellular transformation and tumor metastasis.. Our previous study demonstrated that caveolin-1 expression level was positively correlated with the invasive ability of mouse hepatoma Hepa1-6 and Hca-F cells.. However, the role of caveolin-1 in cellular transformation and apoptosis remains undetermined. Article Title: Functional Analyses of Glycyl-tRNA Synthetase Mutations Suggest a Key Role for tRNA-Charging Enzymes in Peripheral Axons Article Snippet: Alternatively, the PCR primers contained flanking Pst I (forward) and Sma I (reverse) restriction sites to allow cloning into the pEGFP-N2 expression vector (BD Biosciences, Palo Alto, CA) in frame with a C-terminal enhanced green fluorescent protein (EGFP) tag. Article Title: Novel Compound Heterozygous Pathogenic Mutations of SLC5A5 in a Chinese Patient With Congenital Hypothyroidism Article Snippet: Similarly, a WT NIS-EGFP fusion protein was expressed in a pEGFP-N2 expression vector (TransGen Biotech) using EcoRI and BamHI restriction sites, and the GFP was tagged downstream to the NIS coding region. Clone Assay:Article Title: Caveolin-1 promotes the transformation and anti-apoptotic ability of mouse hepatoma cells. Article Snippet: Caveolin-1 is a major structural protein of caveolae and plays important roles in signal transduction, cellular transformation and tumor metastasis.. Our previous study demonstrated that caveolin-1 expression level was positively correlated with the invasive ability of mouse hepatoma Hepa1-6 and Hca-F cells.. However, the role of caveolin-1 in cellular transformation and apoptosis remains undetermined. Article Title: Functional Analyses of Glycyl-tRNA Synthetase Mutations Suggest a Key Role for tRNA-Charging Enzymes in Peripheral Axons Article Snippet: Alternatively, the PCR primers contained flanking Pst I (forward) and Sma I (reverse) restriction sites to allow cloning into the pEGFP-N2 expression vector (BD Biosciences, Palo Alto, CA) in frame with a C-terminal enhanced green fluorescent protein (EGFP) tag. Article Title: Novel Compound Heterozygous Pathogenic Mutations of SLC5A5 in a Chinese Patient With Congenital Hypothyroidism Article Snippet: Similarly, a WT NIS-EGFP fusion protein was expressed in a pEGFP-N2 expression vector (TransGen Biotech) using EcoRI and BamHI restriction sites, and the GFP was tagged downstream to the NIS coding region. Labeling:Article Title: Caveolin-1 promotes the transformation and anti-apoptotic ability of mouse hepatoma cells. Article Snippet: Caveolin-1 is a major structural protein of caveolae and plays important roles in signal transduction, cellular transformation and tumor metastasis.. Our previous study demonstrated that caveolin-1 expression level was positively correlated with the invasive ability of mouse hepatoma Hepa1-6 and Hca-F cells.. However, the role of caveolin-1 in cellular transformation and apoptosis remains undetermined. Article Title: Functional Analyses of Glycyl-tRNA Synthetase Mutations Suggest a Key Role for tRNA-Charging Enzymes in Peripheral Axons Article Snippet: Alternatively, the PCR primers contained flanking Pst I (forward) and Sma I (reverse) restriction sites to allow cloning into the pEGFP-N2 expression vector (BD Biosciences, Palo Alto, CA) in frame with a C-terminal enhanced green fluorescent protein (EGFP) tag. Article Title: Novel Compound Heterozygous Pathogenic Mutations of SLC5A5 in a Chinese Patient With Congenital Hypothyroidism Article Snippet: Similarly, a WT NIS-EGFP fusion protein was expressed in a pEGFP-N2 expression vector (TransGen Biotech) using EcoRI and BamHI restriction sites, and the GFP was tagged downstream to the NIS coding region. Transfection:Article Title: Caveolin-1 promotes the transformation and anti-apoptotic ability of mouse hepatoma cells. Article Snippet: Caveolin-1 is a major structural protein of caveolae and plays important roles in signal transduction, cellular transformation and tumor metastasis.. Our previous study demonstrated that caveolin-1 expression level was positively correlated with the invasive ability of mouse hepatoma Hepa1-6 and Hca-F cells.. However, the role of caveolin-1 in cellular transformation and apoptosis remains undetermined. Article Title: Functional Analyses of Glycyl-tRNA Synthetase Mutations Suggest a Key Role for tRNA-Charging Enzymes in Peripheral Axons Article Snippet: Alternatively, the PCR primers contained flanking Pst I (forward) and Sma I (reverse) restriction sites to allow cloning into the pEGFP-N2 expression vector (BD Biosciences, Palo Alto, CA) in frame with a C-terminal enhanced green fluorescent protein (EGFP) tag. Article Title: Novel Compound Heterozygous Pathogenic Mutations of SLC5A5 in a Chinese Patient With Congenital Hypothyroidism Article Snippet: Similarly, a WT NIS-EGFP fusion protein was expressed in a pEGFP-N2 expression vector (TransGen Biotech) using EcoRI and BamHI restriction sites, and the GFP was tagged downstream to the NIS coding region. Mutagenesis:Article Title: Caveolin-1 promotes the transformation and anti-apoptotic ability of mouse hepatoma cells. Article Snippet: Caveolin-1 is a major structural protein of caveolae and plays important roles in signal transduction, cellular transformation and tumor metastasis.. Our previous study demonstrated that caveolin-1 expression level was positively correlated with the invasive ability of mouse hepatoma Hepa1-6 and Hca-F cells.. However, the role of caveolin-1 in cellular transformation and apoptosis remains undetermined. Article Title: Functional Analyses of Glycyl-tRNA Synthetase Mutations Suggest a Key Role for tRNA-Charging Enzymes in Peripheral Axons Article Snippet: Alternatively, the PCR primers contained flanking Pst I (forward) and Sma I (reverse) restriction sites to allow cloning into the pEGFP-N2 expression vector (BD Biosciences, Palo Alto, CA) in frame with a C-terminal enhanced green fluorescent protein (EGFP) tag. Article Title: Novel Compound Heterozygous Pathogenic Mutations of SLC5A5 in a Chinese Patient With Congenital Hypothyroidism Article Snippet: Similarly, a WT NIS-EGFP fusion protein was expressed in a pEGFP-N2 expression vector (TransGen Biotech) using EcoRI and BamHI restriction sites, and the GFP was tagged downstream to the NIS coding region. Fluorescence:Article Title: Caveolin-1 promotes the transformation and anti-apoptotic ability of mouse hepatoma cells. Article Snippet: Caveolin-1 is a major structural protein of caveolae and plays important roles in signal transduction, cellular transformation and tumor metastasis.. Our previous study demonstrated that caveolin-1 expression level was positively correlated with the invasive ability of mouse hepatoma Hepa1-6 and Hca-F cells.. However, the role of caveolin-1 in cellular transformation and apoptosis remains undetermined. Article Title: Functional Analyses of Glycyl-tRNA Synthetase Mutations Suggest a Key Role for tRNA-Charging Enzymes in Peripheral Axons Article Snippet: Alternatively, the PCR primers contained flanking Pst I (forward) and Sma I (reverse) restriction sites to allow cloning into the pEGFP-N2 expression vector (BD Biosciences, Palo Alto, CA) in frame with a C-terminal enhanced green fluorescent protein (EGFP) tag. Article Title: Novel Compound Heterozygous Pathogenic Mutations of SLC5A5 in a Chinese Patient With Congenital Hypothyroidism Article Snippet: Similarly, a WT NIS-EGFP fusion protein was expressed in a pEGFP-N2 expression vector (TransGen Biotech) using EcoRI and BamHI restriction sites, and the GFP was tagged downstream to the NIS coding region. Staining:Article Title: Caveolin-1 promotes the transformation and anti-apoptotic ability of mouse hepatoma cells. Article Snippet: Caveolin-1 is a major structural protein of caveolae and plays important roles in signal transduction, cellular transformation and tumor metastasis.. Our previous study demonstrated that caveolin-1 expression level was positively correlated with the invasive ability of mouse hepatoma Hepa1-6 and Hca-F cells.. However, the role of caveolin-1 in cellular transformation and apoptosis remains undetermined. Article Title: Functional Analyses of Glycyl-tRNA Synthetase Mutations Suggest a Key Role for tRNA-Charging Enzymes in Peripheral Axons Article Snippet: Alternatively, the PCR primers contained flanking Pst I (forward) and Sma I (reverse) restriction sites to allow cloning into the pEGFP-N2 expression vector (BD Biosciences, Palo Alto, CA) in frame with a C-terminal enhanced green fluorescent protein (EGFP) tag. Article Title: Novel Compound Heterozygous Pathogenic Mutations of SLC5A5 in a Chinese Patient With Congenital Hypothyroidism Article Snippet: Similarly, a WT NIS-EGFP fusion protein was expressed in a pEGFP-N2 expression vector (TransGen Biotech) using EcoRI and BamHI restriction sites, and the GFP was tagged downstream to the NIS coding region. Polymerase Chain Reaction:Article Title: Caveolin-1 promotes the transformation and anti-apoptotic ability of mouse hepatoma cells. Article Snippet: Caveolin-1 is a major structural protein of caveolae and plays important roles in signal transduction, cellular transformation and tumor metastasis.. Our previous study demonstrated that caveolin-1 expression level was positively correlated with the invasive ability of mouse hepatoma Hepa1-6 and Hca-F cells.. However, the role of caveolin-1 in cellular transformation and apoptosis remains undetermined. Article Title: Functional Analyses of Glycyl-tRNA Synthetase Mutations Suggest a Key Role for tRNA-Charging Enzymes in Peripheral Axons Article Snippet: Alternatively, the PCR primers contained flanking Pst I (forward) and Sma I (reverse) restriction sites to allow cloning into the pEGFP-N2 expression vector (BD Biosciences, Palo Alto, CA) in frame with a C-terminal enhanced green fluorescent protein (EGFP) tag. Article Title: Novel Compound Heterozygous Pathogenic Mutations of SLC5A5 in a Chinese Patient With Congenital Hypothyroidism Article Snippet: Similarly, a WT NIS-EGFP fusion protein was expressed in a pEGFP-N2 expression vector (TransGen Biotech) using EcoRI and BamHI restriction sites, and the GFP was tagged downstream to the NIS coding region. Purification:Article Title: Caveolin-1 promotes the transformation and anti-apoptotic ability of mouse hepatoma cells. Article Snippet: Caveolin-1 is a major structural protein of caveolae and plays important roles in signal transduction, cellular transformation and tumor metastasis.. Our previous study demonstrated that caveolin-1 expression level was positively correlated with the invasive ability of mouse hepatoma Hepa1-6 and Hca-F cells.. However, the role of caveolin-1 in cellular transformation and apoptosis remains undetermined. Article Title: Functional Analyses of Glycyl-tRNA Synthetase Mutations Suggest a Key Role for tRNA-Charging Enzymes in Peripheral Axons Article Snippet: Alternatively, the PCR primers contained flanking Pst I (forward) and Sma I (reverse) restriction sites to allow cloning into the pEGFP-N2 expression vector (BD Biosciences, Palo Alto, CA) in frame with a C-terminal enhanced green fluorescent protein (EGFP) tag. Article Title: Novel Compound Heterozygous Pathogenic Mutations of SLC5A5 in a Chinese Patient With Congenital Hypothyroidism Article Snippet: Similarly, a WT NIS-EGFP fusion protein was expressed in a pEGFP-N2 expression vector (TransGen Biotech) using EcoRI and BamHI restriction sites, and the GFP was tagged downstream to the NIS coding region. Agarose Gel Electrophoresis:Article Title: Caveolin-1 promotes the transformation and anti-apoptotic ability of mouse hepatoma cells. Article Snippet: Caveolin-1 is a major structural protein of caveolae and plays important roles in signal transduction, cellular transformation and tumor metastasis.. Our previous study demonstrated that caveolin-1 expression level was positively correlated with the invasive ability of mouse hepatoma Hepa1-6 and Hca-F cells.. However, the role of caveolin-1 in cellular transformation and apoptosis remains undetermined. Article Title: Functional Analyses of Glycyl-tRNA Synthetase Mutations Suggest a Key Role for tRNA-Charging Enzymes in Peripheral Axons Article Snippet: Alternatively, the PCR primers contained flanking Pst I (forward) and Sma I (reverse) restriction sites to allow cloning into the pEGFP-N2 expression vector (BD Biosciences, Palo Alto, CA) in frame with a C-terminal enhanced green fluorescent protein (EGFP) tag. Article Title: Novel Compound Heterozygous Pathogenic Mutations of SLC5A5 in a Chinese Patient With Congenital Hypothyroidism Article Snippet: Similarly, a WT NIS-EGFP fusion protein was expressed in a pEGFP-N2 expression vector (TransGen Biotech) using EcoRI and BamHI restriction sites, and the GFP was tagged downstream to the NIS coding region. |